Journal: Cancers

Article Title: Netrin Family Genes as Prognostic Markers and Therapeutic Targets for Clear Cell Renal Cell Carcinoma: Netrin-4 Acts through the Wnt/β-Catenin Signaling Pathway

doi: 10.3390/cancers15102816

Figure Lengend Snippet: Listed below are the primary antibodies used in this research.

Article Snippet: p-GSK3β , Rabbit , 1:800 , − , − , Proteintech.

Techniques:

Journal: Cell metabolism

Article Title: Abrogating mitochondrial dynamics in mouse hearts accelerates mitochondrial senescence

doi: 10.1016/j.cmet.2017.09.023

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyconal anti-LC3 A/B , Cell Signaling Technology , Cat# 4108.

Techniques: Virus, Plasmid Preparation, Recombinant, TUNEL Assay, Software

Journal: Environmental Science and Pollution Research International

Article Title: The impact of organic extracts of seasonal PM 2.5 on primary human lung epithelial cells and their chemical characterization

doi: 10.1007/s11356-021-14850-1

Figure Lengend Snippet: The effects of PM 2.5 on the expression of aging and macroautophagy markers in BEAS-2B cells. Cells were exposed to V.C. or PM 2.5 organic compounds (1%) for 24 h. Total cell lysates were extracted and then subjected to western blot analysis for p16, p21, p27, LC3B, and GAPDH. Densitometry analysis was performed using Scion image software

Article Snippet: Rabbit polyclonal anti-phospho-p44/42 MAPK (Thr202/Tyr204) (p-ERK), anti-ERK, and anti-light chain 3B (LC3B) antibodies, and U0126 (a highly selective inhibitor of MEK1 and MEK2) were obtained from Cell Signaling (Danvers, MA, USA).

Techniques: Expressing, Western Blot, Software

Journal: Environmental Science and Pollution Research International

Article Title: The impact of organic extracts of seasonal PM 2.5 on primary human lung epithelial cells and their chemical characterization

doi: 10.1007/s11356-021-14850-1

Figure Lengend Snippet: The effects of PM 2.5 on the expression of aging and autophagy markers in primary HAECs. Primary HAECs (n = 6) were exposed to V.C. or PM 2.5 organic compounds (1%) for 24 h. Total cell lysates were extracted and then subjected to Western blot analysis for p16, p21, p27, LC3B, and GAPDH ( a ). Densitometry analysis using Scion image software ( b ). Data are presented as the mean ± SE. **p < 0.05

Article Snippet: Rabbit polyclonal anti-phospho-p44/42 MAPK (Thr202/Tyr204) (p-ERK), anti-ERK, and anti-light chain 3B (LC3B) antibodies, and U0126 (a highly selective inhibitor of MEK1 and MEK2) were obtained from Cell Signaling (Danvers, MA, USA).

Techniques: Expressing, Western Blot, Software

Journal: Cellular and Molecular Neurobiology

Article Title: Nitric Oxide Donor Alleviates Cardiac Arrest Induced Blood Brain Barrier Injury by Inhibiting HMGB1-ATG5 Mediated Endothelial Autophagy

doi: 10.1007/s10571-026-01706-w

Figure Lengend Snippet: JS-K attenuates autophagic activity in cerebral microvascular endothelial cells after CA/CPR and OGD/R. A Representative immunoblot images and B quantitation of LC3B II and LAMP2 in bEnd.3 cells from Control, OGD/R and OGD/R + JS-K groups. β-actin was used as the protein loading control. The images of western blotting data derived from triplicate blots conducted as three independent experiments. C The bEnd.3 cells were transfected with RFP-mWasabi-LC3 lentiviral vector in Control, OGD/R, and OGD/R + JS-K groups. Representative images of fluorescent LC3 dots are shown. D Mean number of autophagosomes (dots with both red and green color; i.e., dots with yellow color in merged images) and autolysosomes (dots with only red but not green color; i.e., dots with red color in merged images) per cell. Scale bar = 10 μm. E Representative electron micrographs of bEnd.3 cells in Control, OGD/R and OGD/R + JS-K groups. F The percentage of autophagic vacuoles (white arrows) per cytoplasm area was calculated. Scale bar = 5 μm. G Representative immunoblot images and H quantitation of LC3B II and LAMP2 in cerebral microvessels of mice in Sham, CA/CPR and CA/CPR + JS-K groups at 24 h after ROSC. β-actin was used as the protein loading control. The images of western blotting data derived from triplicate blots conducted as three independent experiments

Article Snippet: Membranes were blocked with 5% skim milk and incubated with primary antibodies against Occludin (13409-1-AP, RRID:AB_2156308, Proteintech), ZO-1 (21773-1-AP, AB_10733242, Proteintech), LC3B II/I (#3868, RRID:AB_2137707, CST), LAMP2 (bs-2379R, RRID:AB_11048043, Bioss, China), HMGB1 (10829-1-AP, RRID:AB_2232989, Proteintech), ATG5 (10181-2-AP, RRID:AB_2062045, Proteintech) and HRP-conjugated secondary antibodies (1:2 000, bs-0302R, Bioss).

Techniques: Activity Assay, Western Blot, Quantitation Assay, Control, Derivative Assay, Transfection, Plasmid Preparation

Journal: Cellular and Molecular Neurobiology

Article Title: Nitric Oxide Donor Alleviates Cardiac Arrest Induced Blood Brain Barrier Injury by Inhibiting HMGB1-ATG5 Mediated Endothelial Autophagy

doi: 10.1007/s10571-026-01706-w

Figure Lengend Snippet: Inhibition of HMGB1 nucleocytoplasmic translocation attenuates autophagy and improves endothelial function in bEnd.3 cells. A Representative immunoblot images and B quantitation of nuclear HMGB1, cytoplasmic HMGB1, LC3B II, LAMP2, occludin and ZO-1 expression in bEnd.3 cells from Control, OGD/R and OGD/R + Leptomycin B groups. Histone H3 and β-actin were used as the protein loading control. The images of western blotting data derived from triplicate blots conducted as three independent experiments. C Representative immunofluorescence images of HMGB1 localization in bEnd.3 cells from Control, OGD/R and OGD/R + Leptomycin B groups. HMGB1 (green), nuclei stained with DAPI (blue). Scale bar = 50 μm. D Quantification of nuclear and cytoplasmic HMGB1 signals. Nuclear HMGB1 was defined as HMGB1 signal overlapping with DAPI, and cytoplasmic HMGB1 as non-overlapping HMGB1 signal. E Representative images of fluorescent LC3 dots are shown. F Mean number of autophagosomes and autolysosomes per cell. Scale bar = 10 μm. G The diffusion rates of FITC-dextran (40 kDa). H Representative images of transwell migration assay and I quantitative analysis of migration cells. Scale bar = 100 μm. J Representative images of tube formation assay and K quantitative analysis of total tube length. Scale bar = 100 μm

Article Snippet: Membranes were blocked with 5% skim milk and incubated with primary antibodies against Occludin (13409-1-AP, RRID:AB_2156308, Proteintech), ZO-1 (21773-1-AP, AB_10733242, Proteintech), LC3B II/I (#3868, RRID:AB_2137707, CST), LAMP2 (bs-2379R, RRID:AB_11048043, Bioss, China), HMGB1 (10829-1-AP, RRID:AB_2232989, Proteintech), ATG5 (10181-2-AP, RRID:AB_2062045, Proteintech) and HRP-conjugated secondary antibodies (1:2 000, bs-0302R, Bioss).

Techniques: Inhibition, Translocation Assay, Western Blot, Quantitation Assay, Expressing, Control, Derivative Assay, Immunofluorescence, Staining, Diffusion-based Assay, Transwell Migration Assay, Migration, Tube Formation Assay

Journal: Cellular and Molecular Neurobiology

Article Title: Nitric Oxide Donor Alleviates Cardiac Arrest Induced Blood Brain Barrier Injury by Inhibiting HMGB1-ATG5 Mediated Endothelial Autophagy

doi: 10.1007/s10571-026-01706-w

Figure Lengend Snippet: Inhibition of ATG5 improves endothelial function and BBB function without affecting HMGB1 nucleocytoplasmic translocation. A Representative immunoblot images and B quantitation of nuclear HMGB1, cytoplasmic HMGB1, ATG5, LC3B II, LAMP2, occludin and ZO-1 expression in bEnd.3 cells from Control, OGD/R and OGD/R + ATG5 siRNA groups. Histone H3 and β-actin were used as the protein loading control. The images of western blotting data derived from triplicate blots conducted as three independent experiments. C Representative immunofluorescence images of HMGB1 localization in bEnd.3 cells from Control, OGD/R and OGD/R + ATG5 siRNA groups. HMGB1 (green), nuclei stained with DAPI (blue). Scale bar = 50 μm. D Quantification of nuclear and cytoplasmic HMGB1 signals. Nuclear HMGB1 was defined as HMGB1 signal overlapping with DAPI, and cytoplasmic HMGB1 as non-overlapping HMGB1 signal. E Representative images of tube formation assay and F quantitative analysis of total tube length. Scale bar = 100 μm. G Representative images of transwell migration assay and H quantitative analysis of migration cells. Scale bar = 100 μm. I Representative immunoblot images and J quantitation of nuclear HMGB1, cytoplasmic HMGB1, ATG5, LC3B II, LAMP2, occludin and ZO-1 expression in cerebral microvessels of mice from Sham, CA/CPR and CA/CPR + shATG5 groups at 24 h after ROSC. Histone H3 and β-actin were used as the protein loading control. The images of western blotting data derived from triplicate blots conducted as three independent experiments. K Representative immunofluorescence images of occludin and CD31 co-staining in brain sections at 24 h after ROSC and L Representative immunofluorescence images of ZO-1 and CD31 co-staining in brain sections at 24 h after ROSC and M quantification of occludin and CD31 merged area. Scale bars: main panel = 20 μm; zoom-in = 10 μm. N quantification of ZO-1 and CD31 merged area. Scale bars: main panel = 20 μm; zoom-in = 10 μm. O BBB permeability evaluated using EB at 24 h after ROSC. P Brain water content of mice at 24 h after ROSC

Article Snippet: Membranes were blocked with 5% skim milk and incubated with primary antibodies against Occludin (13409-1-AP, RRID:AB_2156308, Proteintech), ZO-1 (21773-1-AP, AB_10733242, Proteintech), LC3B II/I (#3868, RRID:AB_2137707, CST), LAMP2 (bs-2379R, RRID:AB_11048043, Bioss, China), HMGB1 (10829-1-AP, RRID:AB_2232989, Proteintech), ATG5 (10181-2-AP, RRID:AB_2062045, Proteintech) and HRP-conjugated secondary antibodies (1:2 000, bs-0302R, Bioss).

Techniques: Inhibition, Translocation Assay, Western Blot, Quantitation Assay, Expressing, Control, Derivative Assay, Immunofluorescence, Staining, Tube Formation Assay, Transwell Migration Assay, Migration, Permeability